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Registros recuperados: 15
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Bases biologiques de la production de deux Mollusques d'intérêt économique: reproduction chez Megathura crenulata, reproduction et développement chez Crassostrea gigas ArchiMer
Cannuel, Rozenn.
The reproductive cycle of Megathura crenulata was documented and quantification of oocyte lipid reserves with lipo-specific stains was explored in both this species and in Crassostrea gigas. The necessity of providing food to female broodstock C. gigas in spring was re-evaluated, because subsequent larval and post-larval rearing performance was not affected by broodstock starvation during conditioning. Developmental study of the organs responsible of particle processing in C. gigas allowed the identification of critical stages related to the ontogenesis of each organ, as well as a better understanding of the evolution of these organs, highly-modified in the Ostreidae. Ciliary type identification and mucocyte mapping from post-larval to adult stage showed...
Tipo: Text Palavras-chave: Mucocytes; Mantle; Gill; Larvae postlarvae juveniles; Feeding; Crassostrea gigas; Megathura crenulata; Development; Reproduction; Mucocytes; Manteau; Branchie; Larves post larves juvéniles; Alimentation; Crassostrea gigas; Megathura crenulata; Développement; Reproduction.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/these-1371.pdf
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Ecophysiologie de la reproduction chez l'huître creuse, Crassostrea gigas ArchiMer
Pouvreau, Stephane; Lepennec, M.
The reproductive cycle of Megathura crenulata was documented and quantification of oocyte lipid reserves with lipo-specific stains was explored in both this species and in Crassostrea gigas. The necessity of providing food to female broodstock C. gigas in spring was re-evaluated, because subsequent larval and post-larval rearing performance was not affected by broodstock starvation during conditioning. Developmental study of the organs responsible of particle processing in C. gigas allowed the identification of critical stages related to the ontogenesis of each organ, as well as a better understanding of the evolution of these organs, highly-modified in the Ostreidae. Ciliary type identification and mucocyte mapping from post-larval to adult stage showed...
Tipo: Text Palavras-chave: Mucocytes; Mantle; Gill; Larvae postlarvae juveniles; Feeding; Crassostrea gigas; Megathura crenulata; Development; Reproduction; Mucocytes; Manteau; Branchie; Larves post larves juvéniles; Alimentation; Crassostrea gigas; Megathura crenulata; Développement; Reproduction.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/rapport-1867.pdf
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Effect of salinity and temperature on the expression of genes involved in branchial ion transport processes in European sea bass ArchiMer
Masroor, Waliullah; Farcy, Emilie; Blondeau-bidet, Eva; Venn, Alexander; Tambutté, Eric; Lorin-nebel, Catherine.
The responses of European sea bass to temperature increase and salinity decrease were investigated measuring mRNA expression levels of main genes involved in ion transport. Juvenile fish were pre-acclimated to seawater (SW) at 18 °C (temperate) or 24 °C (warm) for two weeks and then transferred for two weeks to either fresh water (FW) or SW at the respective temperature. Unlike temperate conditions, there is no change in Na+/K+-ATPase α1a (nka α1a) and Na+/H+ exchanger 3 (nhe3) mRNA expression following FW transfer in warm conditions. This is linked to the high expression of these genes in warm SW compared to temperate SW. Na+/Cl−-cotransporter (ncc2a) expression however is increased following FW transfer in temperate and warm conditions. Main transporters...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Ion transporter; Gill; MRNA expression; Thermal acclimation; Salinity.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00514/62600/66958.pdf
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Effects of Biodegradable Detergents in the Accumulation of Lipofuscin (Age Pigment) in Gill and Liver of Two Neotropical Fish Species International Journal of Morphology
Pereira,Bruno Fiorelini; Alves,Anderson Luis; Senhorini,José Algusto; Rocha,Rita de Cássia Gimenes de Alcântara; Pitol,Dimítrius Leonardo; Caetano,Flávio Henrique.
Currently there is a growing concern, in both population and governments, to identify the effects of substances commonly disposed of into rivers and lakes, on aquatic fauna and flora. Thus the objective of the present study was to verify effects of biodegradable detergents and water from an urban lake on gills and liver of two neotropical fish species of great economic importance, Astyanax altiparanae and Prochilodus lineatus. In order to do so, lipofuscin, also called the ageing pigment, was used as bioindicator. After one and five months of experiment both tissues accumulated this pigment. These data are discussed from physiological points of view, related with lipid peroxidation and mitochondrial damage.
Tipo: Journal article Palavras-chave: Lipofuscin; Liver; Gill; Prochilodus; Astyanax.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000300005
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Effects of the organophosphorous methyl parathion on the branchial epithelium of a freshwater fish Metynnis roosevelti BABT
Machado,Marcelo Rubens; Fanta,Edith.
Gills are vital structures for fish, since they are the main site for gaseous exchange as well as partially responsible for osmorregulation, acid-basic balance, excretion of nitrogenous compounds and taste. Chemicals in the water may alter the morphology of branchial cells of fish that are, therefore, a useful model for environmental impact and ecotoxicology studies. In order to investigate the effects of an organophosphorous compound, methyl parathion, on the gills of the fish, samples of Metynnis roosevelti were exposed to lethal (7ppm) and sublethal (1ppm) doses of Mentox 600 CE. Through light and scanning electron microscopy, shrinking of the branchial epithelium, followed by detachment and hyperplasia were observed. Externally, the branchial filaments...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gill; Fish; Methyl parathion; Toxicity; Morphology.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300008
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Gill and Liver Histopathology in Goodea atripinnis Jordan, Related to Oxidative Stress in Yuriria Lake, Mexico International Journal of Morphology
Ruiz-Picos,R; López-López,E.
In aquatic ecosystems, the complex mixture of pollutants may mediate the formation of free radicals and cause oxidative damage to the biota. Yuriria Lake (a Ramsar site in Central Mexico) receives input of wastewater from its tributaries, agricultural runoff, and municipal discharge. We studied the lipid peroxidation (LPO), antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), and histopathology of gill and liver of the native fish Goodea atripinnis in Yuriria Lake. Results were compared to a control group of fish cultivated in the laboratory. LPO, SOD, and CAT showed no significant differences compared to controls, but GPx showed greater and significant differences in both tissues. Three class sizes were...
Tipo: Journal article Palavras-chave: Mexican wetlands; Oxidative stress; Histopathology; Fish health condition; Liver; Gill.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300060
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"Gill disease" of Portuguese Oysters ArchiMer
Alderman, D. J.; Gras, Paul.
During April and May of this year, there has been a heavy mortality of Portuguese oysters, Crassostrea angulata LmK., which were imported into Britain from the River Tagus (Portugal) in March. Losses in some cases exceeded 90 per cent. The symptoms shown by the dyring oysters are the same as those seen in Britain and France in C. angulata in 1967 and 1968. Farley described the general symptoms in oysters (C. virginica Gmelin) dying of prolonged functional or infectious disease. These gross symtoms appear to be similar in the case of several different diseases including the present European mortality.
Tipo: Text Palavras-chave: Gill; Crassostrea angulata; Oysters.
Ano: 1969 URL: http://archimer.ifremer.fr/doc/1969/publication-5931.PDF
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Gill histopathological alterations in Nile tilapia, Oreochromis niloticus exposed to treated sewage Water BABT
Fontaínhas-Fernandes,António; Luzio,Ana; Garcia-Santos,Sofia; Carrola,João; Monteiro,Sandra.
Adult Nile tilapia, Oreochromis niloticus, of both sexes were exposed in wastewater from a sewage treatment plant for a period of 4 days. Gill samples were collected after 24, 48, 72 and 96 h and histopathological changes were analyzed by light and scanning electronic microscopy. Gill epithelium of control O. niloticus (freshwater group) was similar to that of other teleosts, while histopathological lesions were observed in exposed fishes. The main histopathological changes were edema, lifting of lamellar and filamentar epithelia and lamellar fusion. Cell proliferation with consequent thickening of the filament epithelium was also found in fishes exposed to the treated sewage water. The severity of the lesions increased with the time of exposure, namely...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gill; Tilapia; Histopathology; Wastewater.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500023
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Gill transcriptomic analysis in fast- and slow-growing individuals of Mytilus galloprovincialis ArchiMer
Prieto, Daniel; Markaide, Pablo; Urrutxurtu, Iñaki; Navarro, Enrique; Artigaud, Sebastien; Fleury, Elodie; Ibarrola, Irrintzi; Urrutia, Miren Bego.
The molecular basis underlying the mechanisms at the origin of growth variation in bivalves is still poorly understood, although several genes have been described as upregulated in fast-growing individuals. In the present study, we reared mussel spat of the species Mytilus galloprovincialis under diets below the pseudofaeces threshold (BP) and above the pseudofaeces threshold (AP). After 3 months, F and S mussels from each condition were selected to obtain 4 experimental groups: FBP, SBP, FAP and SAP. We hypothesized that the nurturing conditions during the growing period would modify the molecular basis of their growth rate differences. To decipher the molecular mechanisms underlying the growth variation, the gill transcriptomes for the four mussel groups...
Tipo: Text Palavras-chave: Fast-growing; Mussel; Gill; Transcriptome.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00503/61490/65293.pdf
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Gills scanning images of the seawater fish Eugerres brasilianus (Gerreidae) BABT
Eiras-Stofella,Daura Regina; Charvet-Almeida,Patricia.
The gills of the adult fish, Eugerres brasilianus (Gerreidae) were analyzed in a scanning electron microscope. The stratified epithelium was uniform on all parts of the branchial arch. Concentric microridges were present on cells that form this epithelium and were mainly observed in the primary lamellae and pharyngeal region where mucous cells were also abundant. The ultrastructural features of E. brasilianus gills indicated that this was not a filtering species, and that the feeding habit included mainly the intake of small organisms. The results presently obtained agreed with other literature data which determined the feeding habit of this species by means of stomach content analysis and other aspects.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gill; Morphology; Scanning; Eugerres brasilianus.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400011
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Immune priming and portal of entry effectors improve response to vibrio infection in a resistant population of the European abalone ArchiMer
Dubief, Bruno; Nunes, Flavia; Basuyaux, Olivier; Paillard, Christine.
Since 1997, populations of the European abalone Haliotis tuberculata suffer mass mortalities attributed to the bacterium Vibrio harveyi. These mortalities occur at the spawning season, when the abalone immune system is depressed, and when temperatures exceed 17 °C, leading to favorable conditions for V. harveyi proliferation. In order to identify mechanisms of disease resistance, experimental successive infections were carried out on two geographically distinct populations: one that has suffered recurrent mortalities (Saint-Malo) and one that has not been impacted by the disease (Molène). Furthermore, abalone surviving these two successive bacterial challenges and uninfected abalone were used for several post-infection analyses. The Saint-Malo population...
Tipo: Text Palavras-chave: Immunity; Hemocyte; Abalone; Disease; Extracellular products; Immune priming; Vibrio harveyi; Flow cytometry; Resistance; Phagocytosis; Bacterial growth; QPCR; Gill.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00357/46800/46667.pdf
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Isolation and primary culture of gill and digestive gland cells from the common mussel Mytilus edulis ArchiMer
Faucet, Jerome; Maurice, Manuelle; Gagnaire, Beatrice; Renault, Tristan; Burgeot, Thierry.
As the marine mussel Mytilus edulis is commonly used as a sentinel species, it would be useful to develop a primary culture of the target organs most often in contact with the marine environment. This study reports an improved method for dissociating the digestive gland and gills of M. edulis and considers the effect of mussel storage on cell viability and functionality before culture initiation. Viability and enzymatic activities such as those of esterase and peroxidase were monitored by flow cytometry, a sensitive, objective technique allowing large volumes of cells to be counted within a short time. A primary culture of digestive gland showed more than 75% viability after 72 h. Mussels were maintained in an aquarium containing clean, oxygenated seawater...
Tipo: Text Palavras-chave: Mytilus edulis; Gill; Flow cytometry; Digestive gland; Cell culture.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-936.pdf
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Molecular characterisation of prolactin and analysis of extrapituitary expression in the European sea bass Dicentrarchus labrax under various salinity conditions ArchiMer
Boutet, Isabelle; Lorin Nebel, C; De Lorgeril, Julien; Guinand, B.
dAlthough prolactin has been demonstrated to be the main hormone involved in adaptation to dilute media in several freshwater teleosts, few studies have been conducted in marine teleosts. In the Mediterranean, the sea bass Dicentrarchus labrax inhabits environments ranging from the open sea to coastal lagoons, where salinity varies greatly. We characterised the prolactin (prl) gene and analysed its expression in two organs (gill and intestine) in D. labrax acclimated to either freshwater or seawater. A 2819 bp long sequence encompassing the prl gene and a part (282 bp) of the promoter were identified, and these comprised 5 coding exons separated by 4 introns. Prolactin was similarly expressed in fresh- and seawater adapted fish, although expression in...
Tipo: Text Palavras-chave: Prolactin; Phylogenctic analysis; MRNA expression; Osmoregulation; Intestine; Gill; Dicentrarchuslabrax.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2537.pdf
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Peppermint essential oil as an anesthetic for and toxicity to juvenile silver catfish PAB
Spanghero,Diogo Bessa Neves; Spanghero,Emília Carolina Alencar de Medeiros; Pedron,Janaína dos Santos; Chagas,Edsandra Campos; Chaves,Francisco Célio Maia; Zaniboni-Filho,Evoy.
Abstract: The objective of this work was to evaluate peppermint (Mentha piperita) essential oil toxicity to and potential as an anesthetic for juvenile silver catfish (Rhamdia quelen). To determine the lethal concentration at 4 hours (LC50-4h), 210 fish (3.08±0.8 g and 7.59±0.67 cm) were exposed to 0, 20, 50, 80, 110, and 140 mg L-1 essential oil. To evaluate the anesthetic potential, nine fish were individually exposed to each oil concentration (50, 80, 110, and 140 mg L-1) used. Water quality parameters were controlled. The mortality rate and the severity and extent of the gill injuries of silver catfish juveniles at 4 hours of exposure increased with increasing peppermint essential oil concentrations, with LC50-4h estimated to be 75.06 mg L-1. The main...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mentha piperita; Rhamdia quelen; Anesthesia; Gill; Histology; Lethal concentration.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000101102
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Spatial compartmentalization of free radical formation and mitochondrial heterogeneity in bivalve gills revealed by live-imaging techniques ArchiMer
Rivera-ingraham, Georgina A.; Rocchetta, Iara; Bickmeyer, Ulf; Meyer, Stefanie; Abele, Doris.
Background: Reactive oxygen (ROS) and nitrogen (RNS) species are produced during normal unstressed metabolic activity in aerobic tissues. Most analytical work uses tissue homogenates, and lacks spatial information on the tissue specific sites of actual ROS formation. Live-imaging techniques (LIT) utilize target-specific fluorescent dyes to visualize biochemical processes at cellular level. Results: Together with oxidative stress measurements, here we report application of LIT to bivalve gills for ex-vivo analysis of gill physiology and mapping of ROS and RNS formation in the living tissue. Our results indicate that a) mitochondria located in the basal parts of the epithelial cells close to the blood vessels are hyperpolarized with high Delta Psi m, whereas...
Tipo: Text Palavras-chave: Bivalve; Gill; Live-imaging; Fluorescence; Mitochondria; ROS; RNS.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00626/73808/74412.pdf
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